In vast agricultural landscapes where dense tree cover is rare, new research is showing just how powerful a strip of trees along a stream can be. Scientists have found that planting and preserving vegetation beside waterways doesn’t just protect water quality — it brings wildlife back.
A study from the University of Illinois reveals that every 10% increase in trees and shrubs along rivers and streams is linked to roughly one additional land species in the area. These vegetated zones, known as riparian buffers, are proving to be quiet but crucial biodiversity boosters.
DNA in Water Reveals Hidden Wildlife
To reach their conclusions, researchers used an innovative method called environmental DNA (eDNA) metabarcoding. By collecting water samples from streams, they analyzed tiny traces of genetic material left behind by animals moving through the environment.
The results were striking. The team detected bobcats, bats, box turtles, and other species — including bobcats, which were once hunted to near-extinction in the Midwest but have been steadily recovering since the 1990s.
“We detected bobcats that have had a big population recovery in Illinois,” said Eric Larson, who leads the research lab. “We detected bats. We detected box turtles. Those benefits to wildlife also have benefits to people.”
Larson explained that this type of sampling is simple enough that farmers or volunteers could collect water samples themselves. That means landowners could directly measure whether conservation efforts, like planting riparian buffers, are actually improving biodiversity on their property.
Study lead author Olivia Reves hopes the findings encourage more widespread adoption of buffers.
“We have really interesting biodiversity in this area,” she said. “We’re doing our best to give these organisms a voice and a fighting chance in this landscape.”
How Buffer Laws Are Shaping Conservation
Some U.S. states already require riparian buffers by law. Minnesota, California, and Alaska have regulations mandating vegetation along public lakes, rivers, and streams. In Minnesota, for example, the Buffer Law requires at least 30 feet of perennial vegetation along public waterways and 16.5 feet along public drainage ditches to reduce runoff and prevent erosion.
When the law passed in 2015, many farmers initially pushed back. But amendments have since introduced flexibility for areas where buffers are difficult to implement.
Tom Gile, a resource conservation manager with Minnesota’s Board of Water and Soil Resources, says compliance has dramatically improved.
“As of today, we sit over 99% compliant with the law,” Gile said.
He added that areas with buffers tend to have cleaner water and more wildlife, even if it’s difficult to measure exactly how much of the improvement comes directly from the buffers.
“It helps keep more sediment out of drainage systems,” he explained. “And it provides additional habitat along these corridors.”
As more evidence emerges, researchers believe riparian buffers could become one of the simplest and most effective tools for restoring wildlife in heavily farmed landscapes.

